HMGCL Polyklonaler Antikörper

HMGCL Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 16898-1-AP

Synonyme

HL, HMG CoA lyase, HMGCL

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Galerie der Validierungsdaten

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  WB analysis using 16898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16898-1-AP (HMGCL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 16898-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16898-1-AP (HMGCL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16898-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16898-1-AP (HMGCL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 16898-1-AP

  IP result of anti-HMGCL (IP:16898-1-AP, 4ug; Detection:16898-1-AP 1:500) with mouse spleen tissue lysate 4000 ug.

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  IHC staining of human liver using 16898-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16898-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 16898-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16898-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16898-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 16898-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 16898-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 16898-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, humanes Herzgewebe, Maushirngewebe, Rattenlebergewebe
• Erfolgreiche IP: Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Eierstockgewebe, humanes Hodengewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Produktinformation

16898-1-AP bindet in WB, IP, IHC, ELISA HMGCL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HMGCL fusion protein Ag10426
• Vollständiger Name: 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
• Berechnetes Molekulargewicht: 325 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC010570
• Gene symbol: HMGCL
• Gene ID (NCBI): 3155
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IHC	Nat Cell Biol; BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.; Authors - Jin-Tao Li
human	WB	Cell Res; Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.; Authors - De Huang
human	WB,IHC	Hepatol Int; HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility; Authors - Xiaohan Cui
mouse	WB	J Biol Chem; Innate immune cell-intrinsic ketogenesis is dispensable for organismal metabolism and age-related inflammation; Authors - Emily L Goldberg
human,mouse	WB,IHC,IP	Int J Biol Sci; Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer; Authors - Chenxi Zhong ; KD Validated
mouse	WB	Cell Rep; Hepatic palmitoyl-proteomes and acyl-protein thioesterase protein proximity networks link lipid modification and mitochondria; Authors - Sarah L Speck