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HMGB3 Polyklonaler Antikörper
HMGB3 Polyklonal Antikörper für IF, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
WB, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 27465-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | BGC-823-Zellen, humanes Plazenta-Gewebe, MCF-7-Zellen, SGC-7901-Zellen |
| Erfolgreiche Detektion in IF | MCF-7-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunfluoreszenz (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
Produktinformation
27465-1-AP bindet in WB, IF, ELISA HMGB3 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | HMGB3 fusion protein Ag26361 |
| Vollständiger Name | high-mobility group box 3 |
| Berechnetes Molekulargewicht | 200 aa, 23 kDa |
| Beobachtetes Molekulargewicht | 25 kDa |
| GenBank-Zugangsnummer | BC070482 |
| Gene symbol | HMGB3 |
| Gene ID (NCBI) | 3149 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
HMGB3, also named HMG2A and HMG4, belongs to the HMGB family. HMGB3 binds to nucleosomes in a sequence-independent manner and participates in DNA repair, replication, transcription, and recombination. HMGB3 is highly expressed in stem cells and cancer cells and is rarely transactivated in normal adult tissues, making it a promising therapeutic target. Overexpressed HMGB3 is closely related to tumor occurrence and reduced survival in cervical cancer, non-small-cell lung cancer, breast cancer, bladder cancer, hepatocellular carcnoma, colorectal cancer, gastric cancer, and leukemia (PMID: 35332131).
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for HMGB3 antibody 27465-1-AP | Protokoll herunterladen |
| IF protocol for HMGB3 antibody 27465-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol HMGB3 is a Potential Therapeutic Target by Affecting the Migration and Proliferation of Colorectal Cancer.
| ||
Genes (Basel) Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.
| ||
Int J Gen Med Circular RNA 0001313 Knockdown Suppresses Non-Small Cell Lung Cancer Cell Proliferation and Invasion via the microRNA-452/HMGB3/ERK/MAPK Axis. | ||
Front Oncol Loss of exosomal micro-RNA-200b-3p from hypoxia cancer-associated fibroblasts reduces sensitivity to 5-flourouracil in colorectal cancer through targeting high-mobility group box 3 | ||
Curr Med Sci Overexpression of MiR-633 Suppresses the Tumorigenicity of Gastric Cancer Cells and Induces Apoptosis by Targeting MAPK1 |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ting (Verified Customer) (10-18-2023) | The Antibody looked bad. I saw many non-specific bands and two bands near the HMGB3 molecular weight when I use this antibody. It is really hard to distinguish the bands, especially for Wild-type group.
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