SPP Polyklonaler Antikörper

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• KD/KO Validated

SPP Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 20416-1-AP

Synonyme

H13, hIMP1, histocompatibility (minor) 13, HM13, IMP 1, IMP1, IMPAS, IMPAS 1, Intramembrane protease 1, MSTP086, Presenilin like protein 3, PSENL3, PSL3, Signal peptide peptidase, SPP

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 20416-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20416-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20416-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 20416-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 20416-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20416-1-AP (SPP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 20416-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20416-1-AP (SPP antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mauslebergewebe, Mausnierengewebe, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

20416-1-AP bindet in WB, IHC, ELISA SPP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPP fusion protein Ag14158
• Vollständiger Name: histocompatibility (minor) 13
• Berechnetes Molekulargewicht: 377 aa, 41 kDa
• Beobachtetes Molekulargewicht: 45 kDa, 100 kDa
• GenBank-Zugangsnummer: BC008938
• Gene symbol: HM13
• Gene ID (NCBI): 81502
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
		PLoS One; Visualizing active enzyme complexes using a photoreactive inhibitor for proximity ligation--application on γ-secretase.; Authors - Schedin-Weiss Sophia S
human	IHC,WB	Cell Death Dis; Histocompatibility Minor 13 (HM13), targeted by miR-760, exerts oncogenic role in breast cancer by suppressing autophagy and activating PI3K-AKT-mTOR pathway; Authors - Haiyan Yang ; KD Validated
human	WB	Front Pharmacol; Pan-cancer analysis suggests histocompatibility minor 13 is an unfavorable prognostic biomarker promoting cell proliferation, migration, and invasion in hepatocellular carcinoma; Authors - Jun Liu ; KD Validated