HLA class I ABC Monoklonaler Antikörper

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• KD/KO Validated

HLA class I ABC Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human

Anwendung

IF, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5C5B7

Kat-Nr. : CL488-66013

Synonyme

HLA A, HLA class I ABC, HLAA, HLA-A, MHC class I antigen A*3

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Galerie der Validierungsdaten

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  IF Staining of human tonsillitis using CL488-66013

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66013 (HLA class I ABC antibody) at dilution of 1:50.

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  IF Staining of Raji using CL488-66013

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CoraLite® Plus 488 HLA class I ABC antibody (CL488-66013, Clone: 5C5B7 ) at dilution of 1:200.

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  FC experiment of Raji using CL488-66013

  1X10^6 Raji cells were surface stained with 0.4 ug CoraLite® Plus 488 Anti-Human HLA class I ABC (CL488-66013, Clone:5C5B7) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe, Raji-Zellen
• Erfolgreiche Detektion in FC: Raji-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66013 bindet in IF, FC HLA class I ABC und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HLA class I ABC fusion protein Ag7488
• Vollständiger Name: major histocompatibility complex, class I, A
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 40-44 kDa
• GenBank-Zugangsnummer: BC003069
• Gene symbol: HLA-A
• Gene ID (NCBI): 3105
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)