HLA-F Polyklonaler Antikörper

HLA-F Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55379-1-AP

Synonyme

CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen, HLAF, HLA-F, Leukocyte antigen F, MHC class I antigen F

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Galerie der Validierungsdaten

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  WB analysis of Raji using 55379-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 55379-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 55379-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55379-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55379-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, A431-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

55379-1-AP bindet in WB,ELISA HLA-F und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: major histocompatibility complex, class I, F
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa, 66-70 kDa
• GenBank-Zugangsnummer: NM_018950
• Gene symbol: HLA-F
• Gene ID (NCBI): 3134
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)