HLA class I (HLA-B) Polyklonaler Antikörper

HLA class I (HLA-B) Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 17260-1-AP

Synonyme

AS, HLA B, HLA B 7301, HLA B73, HLA class I (HLA-B), HLAB, HLA-B, HLAC, MHC class I antigen B*7, SPDA1

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Galerie der Validierungsdaten

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  WB analysis using 17260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17260-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17260-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17260-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17260-1-AP

  IP Result of anti-HLA class I (HLA-B) (IP:17260-1-AP, 4ug; Detection:17260-1-AP 1:2000) with HepG2 cells lysate 4000ug.

• 

  IHC staining of human spleen using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human tonsillitis using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 17260-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17260-1-AP

  Immunofluorescent analysis of HepG2 cells using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Eierstockgewebe, humanes Herzgewebe, humanes Lungengewebe, humanes Magenkrebsgewebe, humanes Nierengewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

17260-1-AP bindet in WB, IP, IHC, IF, ELISA HLA class I (HLA-B) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HLA class I (HLA-B) fusion protein Ag10947
• Vollständiger Name: major histocompatibility complex, class I, B
• Berechnetes Molekulargewicht: 362 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC013187
• Gene symbol: HLA-B
• Gene ID (NCBI): 3106
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-B can also react with HLA-A and HLA-C. (PMID: 667938; 3375250)

Publikationen

Species	Application	Title
human	IHC	Gut; Single-cell RNA sequencing reveals intrahepatic and peripheral immune characteristics related to disease phases in HBV-infected patients.; Authors - Chao Zhang
human	IF	Elife; Cell-surface tethered promiscuous biotinylators enable comparative small-scale surface proteomic analysis of human extracellular vesicles and cells.; Authors - Lisa L Kirkemo
human	WB	ACS Chem Biol; Engineering Antibodies Targeting p16 MHC-Peptide Complexes.; Authors - Nicholas J Rettko
human	WB	Cell Death Dis; Exosomal annexin A6 induces gemcitabine resistance by inhibiting ubiquitination and degradation of EGFR in triple-negative breast cancer.; Authors - Ting Li
human	WB	bioRxiv; Secreted HLA Fc-Fusion Profiles Immunopeptidome in Hypoxic PDAC and Cellular Senescence; Authors - Nicholas J Rettko

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (09-16-2020)

This antibody recognises a specific band at the expected MW.

• Applications: Western Blot

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