Histone H2B Polyklonaler Antikörper

Histone H2B Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27740-1-AP

Synonyme

H2B clustered histone 26, H2B type 12, H2B3B, H2BC26, HIST3H2BB, histone cluster 3, H2bb, Histone H2B type 3 B

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Galerie der Validierungsdaten

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  WB analysis using 27740-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 27740-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 27740-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293T-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27740-1-AP bindet in WB, ELISA Histone H2B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Histone H2B fusion protein Ag26901
• Vollständiger Name: histone cluster 3, H2bb
• Beobachtetes Molekulargewicht: 15-20 kDa
• GenBank-Zugangsnummer: BC100855
• Gene symbol: HIST3H2BB
• Gene ID (NCBI): 128312
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST3H2BB is a replication-dependent histone that is a member of the histone H2B family.