HIST2H2AA4 Polyklonaler Antikörper

HIST2H2AA4 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 15302-1-AP

Synonyme

H2A/R, H2AFO, HIST2H2AA, HIST2H2AA3, HIST2H2AA4, histone, histone cluster 2, H2aa4, Histone H2A type 2 A, Histone H2A.2, Histone H2A/o

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Galerie der Validierungsdaten

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  WB analysis of L02 using 15302-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15302-1-AP (HIST2H2AA4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse liver using 15302-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15302-1-AP (HIST2H2AA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15302-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15302-1-AP (HIST2H2AA4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15302-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15302-1-AP (HIST2H2AA4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15302-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15302-1-AP (HIST2H2AA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15302-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15302-1-AP (HIST2H2AA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15302-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15302-1-AP (HIST2H2AA4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 15302-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15302-1-AP (HIST2H2AA4 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, Jurkat-Zellen, K-562-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

15302-1-AP bindet in WB, IHC,ELISA HIST2H2AA4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HIST2H2AA4 fusion protein Ag7560
• Vollständiger Name: histone cluster 2, H2aa4
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC001629
• Gene symbol: HIST2H2AA4
• Gene ID (NCBI): 723790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Cell Physiol Biochem; Effect of Type I Diabetes on the Proteome of Mouse Oocytes.; Authors - Guangjian Jiang