Histone H1.2 Polyklonaler Antikörper

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• KD/KO Validated

Histone H1.2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Kat-Nr. : 19649-1-AP

Synonyme

H1.2, H1F2, HIST1H1C, histone, histone cluster 1, H1c, Histone H1.2, Histone H1d

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 19649-1-AP

  WB result of Histone H1.2 antibody (19649-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Histone H1.2 transfected MCF-7 cells.

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  WB analysis of L02 using 19649-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 19649-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19649-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19649-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19649-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19649-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 19649-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 19649-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 19649-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19649-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 19649-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19649-1-AP (HIST1H1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 19649-1-AP

  IP Result of anti-Histone H1.2 (IP:19649-1-AP, 3ug; Detection:19649-1-AP 1:500) with HeLa cells lysate 1600ug.

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  IHC staining of human ovary tumor using 19649-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19649-1-AP (HIST1H1C antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 19649-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19649-1-AP (HIST1H1C antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 19649-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H1.2 antibody (19649-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 19649-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Histone H1.2 antibody (19649-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, A375-Zellen, humanes Hodengewebe, Jurkat-Zellen, Maus-Thymusgewebe, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 20 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

19649-1-AP bindet in WB, IP, IHC, IF, ELISA Histone H1.2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Histone H1.2 fusion protein Ag7503
• Vollständiger Name: histone cluster 1, H1c
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 32-33 kDa
• GenBank-Zugangsnummer: BC002649
• Gene symbol: HIST1H1C
• Gene ID (NCBI): 3006
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone H1 regulates chromatin competency through its high affinity binding to linker DNA as a structural component. It acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. The calculated molecular weight of Histone H1.2 is 21 kDa, but modified Histone H1.2 is about 32 kDa. (PMID: 18258596)

Publikationen

Species	Application	Title
mouse	WB	Nat Neurosci; Genome-wide distribution of linker histone H1.0 is independent of MeCP2.; Authors - Aya Ito-Ishida
mouse	WB	Nat Commun; Histone functions as a cell-surface receptor for AGEs.; Authors - Masanori Itakura
human	WB	Oncogene; Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.; Authors - Mengyi Lao
human	WB	Oxid Med Cell Longev; The Formation of Melanocyte Apoptotic Bodies in Vitiligo and the Relocation of Vitiligo Autoantigens under Oxidative Stress.; Authors - Jun Tian
human	WB	J Neuroinflammation; New insights into meningitic Escherichia coli infection of brain microvascular endothelial cells from quantitative proteomics analysis.; Authors - Wen-Tong Liu
human	IF	Front Immunol; HIST1H1C Regulates Interferon-β and Inhibits Influenza Virus Replication by Interacting with IRF3.; Authors - Xiaokun Liu