HIP1R Polyklonaler Antikörper

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• KD/KO Validated

HIP1R Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 16814-1-AP

Synonyme

HIP 12, HIP1 related protein, HIP12, HIP1R, HIP3, ILWEQ, KIAA0655

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Galerie der Validierungsdaten

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  WB analysis using 16814-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16814-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16814-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16814-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16814-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16814-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16814-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16814-1-AP

  IP Result of anti-HIP1R (IP:16814-1-AP, 4ug; Detection:16814-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human placenta using 16814-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16814-1-AP (HIP1R antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 16814-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16814-1-AP (HIP1R antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, humanes Lebergewebe, Mausherzgewebe, Mauslungengewebe, Mausnierengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

16814-1-AP bindet in WB, IP, IHC, IF, ELISA HIP1R und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HIP1R fusion protein Ag10353
• Vollständiger Name: huntingtin interacting protein 1 related
• Berechnetes Molekulargewicht: 615 aa, 70 kDa, 120 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC067085
• Gene symbol: HIP1R
• Gene ID (NCBI): 9026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell; Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.; Authors - Kazuhiro Koikawa ; KD Validated
human	WB	Nat Cell Biol; Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapy.; Authors - Yang Gao ; KO Validated
human	WB	Nat Biomed Eng; Inhibiting PD-L1 palmitoylation enhances T-cell immune responses against tumours.; Authors - Han Yao
human	WB,IP,IF	Nat Chem Biol; HIP1R targets PD-L1 to lysosomal degradation to alter T cell-mediated cytotoxicity.; Authors - Huanbin Wang ; KD Validated
human	WB	Sci Adv; The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase.; Authors - Riyad N H Seervai ; KD Validated
human	IHC	J Clin Med; HIP1R Expression and Its Association with PD-1 Pathway Blockade Response in Refractory Advanced NonSmall Cell Lung Cancer: A Gene Set Enrichment Analysis.; Authors - Young Wha Koh