HIP1 Monoklonaler Antikörper

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• KD/KO Validated

HIP1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

3D3G3

Kat-Nr. : 67163-1-Ig

Synonyme

HIP 1, HIP I, HIP1, ILWEQ

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Galerie der Validierungsdaten

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  WB analysis using 67163-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67163-1-Ig

  WB result of HIP1 antibody (67163-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIP1 transfected HeLa cells.

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  WB analysis of pig brain using 67163-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 67163-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67163-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67163-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 67163-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon cancer using 67163-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using HIP1 antibody (67163-1-Ig, Clone: 3D3G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Hausschwein-Hirngewebe, Maushirngewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Kolonkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67163-1-Ig bindet in WB, IHC, IF, ELISA HIP1 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HIP1 fusion protein Ag17982
• Vollständiger Name: huntingtin interacting protein 1
• Berechnetes Molekulargewicht: 1037 aa, 116 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC110545
• Gene symbol: HIP1
• Gene ID (NCBI): 3092
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.