HIF-1 alpha Monoklonaler Antikörper
HIF-1 alpha Monoklonal Antikörper für WB, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human
Anwendung
WB, IP, IHC, IF, ELISA
Konjugation
Unkonjugiert
CloneNo.
1H3C12
Kat-Nr. : 66730-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HeLa-Zellen, mit Cobaltchlorid behandelte HeLa-Zellen, mit Cobaltchlorid behandelte HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 2 publications below |
WB | See 31 publications below |
IHC | See 9 publications below |
IF | See 7 publications below |
IP | See 1 publications below |
Produktinformation
66730-1-Ig bindet in WB, IP, IHC, IF, ELISA HIF-1 alpha und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | HIF-1 alpha fusion protein Ag15198 |
Vollständiger Name | hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
Berechnetes Molekulargewicht | 826 aa, 93 kDa |
Beobachtetes Molekulargewicht | 120 kDa |
GenBank-Zugangsnummer | BC012527 |
Gene symbol | HIF1A |
Gene ID (NCBI) | 3091 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .
Protokolle
Produktspezifische Protokolle | |
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WB protocol for HIF-1 alpha antibody 66730-1-Ig | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation | ||
Theranostics ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system. | ||
Oxid Med Cell Longev Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway. | ||
J Med Chem Potent Inhibition of HIF1α and p300 Interaction by a Constrained Peptide Derived from CITED2. | ||
J Clin Endocrinol Metab Targeting tumor hypoxia inhibits aggressive phenotype of dedifferentiated thyroid cancer |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH balawant (Verified Customer) (12-20-2022) | I have used this antibody for western blot and it is working great
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FH Diane (Verified Customer) (02-02-2021) | incubated over night 4 degrees C. 1:2500 HRP conjugated secondary. Opti-4CN substrate kit for detection. Also tried ICC on cytospins so ran out of trial size before fully optimized but has potential to provide the information that I am needing.
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