HIF-1 alpha Monoklonaler Antikörper

HIF-1 alpha Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1H3C12

Kat-Nr. : 66730-1-Ig

Synonyme

ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF 1α, HIF1, HIF1 ALPHA, HIF-1 alpha, HIF1A, HIF1α, Member of PAS protein 1, MOP1, PASD8



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, mit Cobaltchlorid behandelte HeLa-Zellen, mit Cobaltchlorid behandelte HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66730-1-Ig bindet in WB, IP, IHC, IF, ELISA HIF-1 alpha und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen HIF-1 alpha fusion protein Ag15198
Vollständiger Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Berechnetes Molekulargewicht 826 aa, 93 kDa
Beobachtetes Molekulargewicht 120 kDa
GenBank-ZugangsnummerBC012527
Gene symbol HIF1A
Gene ID (NCBI) 3091
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .

Protokolle

Produktspezifische Protokolle
WB protocol for HIF-1 alpha antibody 66730-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IHC,IF,IP

Adv Sci (Weinh)

Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation

Authors - Huan Li
humanWB

J Immunother Cancer

TSH-TSHR axis promotes tumor immune evasion.

Authors - Zhenghao Wu
humanWB

Theranostics

ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.

Authors - Xiaoxin Zhang
humanWB

Oxid Med Cell Longev

Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway.

Authors - Ziwei Yang
humanWB

J Med Chem

Potent Inhibition of HIF1α and p300 Interaction by a Constrained Peptide Derived from CITED2.

Authors - Xuan Qin
humanWB,IHC

J Clin Endocrinol Metab

Targeting tumor hypoxia inhibits aggressive phenotype of dedifferentiated thyroid cancer

Authors - Ben Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

balawant (Verified Customer) (12-20-2022)

I have used this antibody for western blot and it is working great

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: renal Cancer cell line
FH

Diane (Verified Customer) (02-02-2021)

incubated over night 4 degrees C. 1:2500 HRP conjugated secondary. Opti-4CN substrate kit for detection. Also tried ICC on cytospins so ran out of trial size before fully optimized but has potential to provide the information that I am needing.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1250
  • Cell Tissue Type: Cell lysates glioblastoma primary cells
HIF-1 alpha Antibody Western Blot validation (1:1250 dilution) in Cell lysates glioblastoma primary cells (Cat no:66730-1-Ig)