HES5 Polyklonaler Antikörper

HES5 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 22666-1-AP

Synonyme

bHLHb38, Hairy and enhancer of split 5, HES5, Transcription factor HES 5

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Galerie der Validierungsdaten

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  WB analysis using 22666-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22666-1-AP (HES5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 22666-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22666-1-AP (HES5 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of mouse brain using 22666-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22666-1-AP (HES5 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 22666-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22666-1-AP (HES5 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below

Produktinformation

22666-1-AP bindet in WB, IHC, ELISA HES5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Nerz
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HES5 fusion protein Ag18270
• Vollständiger Name: hairy and enhancer of split 5 (Drosophila)
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC087840
• Gene symbol: HES5
• Gene ID (NCBI): 388585
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HES5, also known as bHLHb38, has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG). HES5 is expressed in fetal heart and brain tumors. HES5 as a transcriptional repressor of genes that require a bHLH protein for their transcription and plays an important role as neurogenesis negative regulator. HES5 is detected with molecular weight of 41 kDa according to the publication (PMID: 24286475).

Publikationen

Species	Application	Title
mouse	WB	Dev Cell; Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells.; Authors - Rani Ojha
human	WB	Cell Rep; Stub1 maintains proteostasis of master transcription factors in embryonic stem cells.; Authors - Md Mahfuz Al Mamun
rat	WB	Front Pharmacol; Danzhi Xiaoyao Powder Promotes Neuronal Regeneration by Downregulating Notch Signaling Pathway in the Treatment of Generalized Anxiety Disorder.; Authors - Chao Liu
mink	WB	Life Sci; Pantothenic acid promotes dermal papilla cell proliferation in hair follicles of American minks via inhibitor of DNA Binding 3/Notch signaling pathway.; Authors - Zhuo Wang
rat	WB	PLoS One; Notch signaling inhibitor DAPT provides protection against acute craniocerebral injury.; Authors - Hong-Mei Zhang
rat	WB	Drug Des Devel Ther; Notch inhibitor can attenuate apparent diffusion coefficient and improve neurological function through downregulating NOX2-ROS in severe traumatic brain injury.; Authors - Hong-Mei Zhang