HDGF2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HDGF2 Polyklonal Antikörper für IF, IHC,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (1)

Kat-Nr. : 15134-1-AP

Synonyme

HDGF 2, HDGF2, HDGFRP2, HRP 2

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Galerie der Validierungsdaten

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  IHC staining of human colon using 15134-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15134-1-AP (HRP 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 15134-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15134-1-AP (HRP 2 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 15134-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15134-1-AP (HRP 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15134-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15134-1-AP (HRP 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

15134-1-AP bindet in WB, IP, IHC, IF, CoIP, chIP, ELISA HDGF2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HDGF2 fusion protein Ag7281
• Vollständiger Name: hepatoma-derived growth factor-related protein 2
• Berechnetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC000755
• Gene symbol: HDGF2
• Gene ID (NCBI): 84717
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Sci Adv; The H3K36me2 writer-reader dependency in H3K27M-DIPG.; Authors - Jia-Ray Yu ; KD Validated
human	WB	Sci Adv; LEDGF and HDGF2 relieve the nucleosome-induced barrier to transcription in differentiated cells.; Authors - Gary LeRoy
mouse	WB,chIP	Nucleic Acids Res; HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.; Authors - Xu Zhu ; KD Validated
human	IF, IP	Nucleic Acids Res; Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination.; Authors - Annika Baude
human	WB	Cell Rep; A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells.; Authors - Judd F Hultquist ; KO Validated
	WB	Placenta; Circ_0001438 participates in the pathogenesis of preeclampsia via the circ_0001438/miR-942/NLRP3 regulatory network.; Authors - Xiaoping Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (12-04-2023)

Works in ICC (nuclear staining) and WB (no non specific bands)

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / SH-SY5Y cells

HDGF2 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Human / SH-SY5Y cells (Cat no:15134-1-AP)