HDDC3 Polyklonaler Antikörper

HDDC3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 21091-1-AP

Synonyme

HD domain containing 3, HD domain containing protein 3, HDDC3, MESH1

---

Galerie der Validierungsdaten

• 

  WB analysis using 21091-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21091-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 21091-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21091-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 21091-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21091-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 21091-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 21091-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21091-1-AP

  IP Result of anti-HDDC3 (IP:21091-1-AP, 4ug; Detection:21091-1-AP 1:500) with HeLa cells lysate 3400ug.

• 

  IHC staining of human prostate cancer using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21091-1-AP (HDDC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21091-1-AP (HDDC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21091-1-AP (HDDC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21091-1-AP (HDDC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21091-1-AP (HDDC3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21091-1-AP (HDDC3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21091-1-AP (HDDC3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21091-1-AP (HDDC3 antibody) at dilution of 1:100 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, HeLa-Zellen, humanes Herzgewebe, Mausherzgewebe, Maushodengewebe, Mauslungengewebe, Mausnierengewebe, Maus-Pankreasgewebe, Rattenherzgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Hirngewebe, humanes Mammakarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

21091-1-AP bindet in WB, IP, IHC, ELISA HDDC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HDDC3 fusion protein Ag15342
• Vollständiger Name: HD domain containing 3
• Berechnetes Molekulargewicht: 179 aa, 20 kDa
• Beobachtetes Molekulargewicht: 22-25 kDa
• GenBank-Zugangsnummer: BC033794
• Gene symbol: HDDC3
• Gene ID (NCBI): 374659
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MESH1, encodes by HDDC3 in human and Q9VAM9 in Drosophila melanogaster, was identified as functional SpoT orthologs in metazoan (PMID: 20818390). MESH1 is a cytosolic NADPH phosphatase that is induced under stress conditions, leading to the NADPH depletion and ferroptosis-a novel form of iron-dependent regulated cell death characterized by lipid peroxidation. Accordingly, MESH1 removal preserves the NADPH level in stressed cells and promotes their ferroptotic survival(PMID: 32462112, PMID: 34294679).

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; MESH1 knockdown triggers proliferation arrest through TAZ repression.; Authors - Tianai Sun