HDAC5-specific Polyklonaler Antikörper

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• KD/KO Validated

HDAC5-specific Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 16166-1-AP

Synonyme

Antigen NY CO 9, HD5, HDAC5, histone deacetylase 5, KIAA0600, NY CO 9

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 16166-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16166-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 16166-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 16166-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 16166-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 16166-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 16166-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16166-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16166-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, fetales humanes Hirngewebe, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Herzgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:100-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 19 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• CoIP: See 3 publications below

Produktinformation

16166-1-AP bindet in WB, IHC, IF, CoIP, ELISA HDAC5-specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone deacetylase 5
• Berechnetes Molekulargewicht: 122 kDa
• Beobachtetes Molekulargewicht: 120-140 kDa
• GenBank-Zugangsnummer: BC051824
• Gene symbol: HDAC5
• Gene ID (NCBI): 10014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone acetylation and deacetylation alternately exposes and occludes DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. This antibody only binds HDAC5. It does not cross-react with other HDACs.

Publikationen

Species	Application	Title
human	WB	Cell; Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.; Authors - Xun Huang
mouse, rat	WB,IHC,IF	Kidney Int; Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.; Authors - Xiaojie Wang
human	WB	Cancer Lett; ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer; Authors - Chen Zhou
human	WB	J Cell Mol Med; Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.; Authors - Shoujie Feng ; KD Validated
rat	WB,IHC	J Cell Mol Med; HDAC4/5-HMGB1 signalling mediated by NADPH oxidase activity contributes to cerebral ischaemia/reperfusion injury.; Authors - He Min M
rat	WB,IF	Biomed Pharmacother; Trichostatin A increases BDNF protein expression by improving XBP-1s/ATF6/GRP78 axis in Schwann cells of diabetic peripheral neuropathy.; Authors - Jiahui An ; KD Validated