HDAC4 Monoklonaler Antikörper

HDAC4 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D2G12

Kat-Nr. : 66838-1-Ig

Synonyme

HA6116, HD4, HDAC, HDAC A, HDAC4, HDACA, histone deacetylase 4, KIAA0288



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:3000
Immunhistochemie (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66838-1-Ig bindet in WB, IP, IHC, IF, ChIP, ELISA HDAC4 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen HDAC4 fusion protein Ag11345
Vollständiger Name histone deacetylase 4
Berechnetes Molekulargewicht 972 aa, 106 kDa
Beobachtetes Molekulargewicht 140 kDa
GenBank-ZugangsnummerBC039904
Gene symbol HDAC4
Gene ID (NCBI) 9759
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

Protokolle

Produktspezifische Protokolle
WB protocol for HDAC4 antibody 66838-1-IgProtokoll herunterladen
IHC protocol for HDAC4 antibody 66838-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Oncogenesis

USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.

Authors - Wei Zhan
humanIF

Virulence

Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.

Authors - Wei Jie
humanIF

J Mol Cell Cardiol

Cellular heterogeneity and immune microenvironment revealed by single-cell transcriptome in venous malformation and cavernous venous malformation.

Authors - Yongyun Li
mouseWB,IP,ChIP

Appl Physiol Nutr Metab

Exercise improves high-fat diet-induced metabolic disorder by promoting HDAC5 degradation through the ubiquitin-proteasome system in skeletal muscle

Authors - Song Huang
  • KD Validated
mouseWB

J Toxicol Sci

Propofol improves ischemia reperfusion-induced liver fibrosis by regulating lncRNA HOXA11-AS

Authors - Jia Luo
  • KD Validated
human,mouseWB

Adv Clin Exp Med

Tasquinimod enhances the sensitivity of ovarian cancer cells to cisplatin by regulating the Nur77-Bcl-2 apoptotic pathway

Authors - Ying Lin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shinford (Verified Customer) (10-26-2023)

In our screening of basic expression in lung, breast, and gastric cancer cell lines, antibody concentrations ranging from 1:1000 to 2000 all displayed clear bands, indicating stable working conditions.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Multiple cell lines of lung cancer, breast cancer, gastric cancer.
HDAC4 Antibody Western Blot validation (1:1000 dilution) in Multiple cell lines of lung cancer, breast cancer, gastric cancer. (Cat no:66838-1-Ig)