HDAC3-specific Polyklonaler Antikörper

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• KD/KO Validated

HDAC3-specific Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16164-1-AP

Synonyme

HD3, HDAC3, HDAC3-specific, histone deacetylase 3, RPD3, RPD3 2, SMAP45

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Galerie der Validierungsdaten

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  WB analysis using 16164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16164-1-AP (HDAC3-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16164-1-AP

  WB result of HDAC3-specific antibody (16164-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDAC3-specific transfected HeLa cells.

• 

  WB analysis of HEK-293 using 16164-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16164-1-AP (HDAC3-specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 16164-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16164-1-AP (HDAC3-specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HEK-293-Zellen, HeLa-Zellen, HL-60-Zellen, humanes Magengewebe, MOLT-4-Zellen, NCI-H1299-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16164-1-AP bindet in WB, ELISA HDAC3-specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone deacetylase 3
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: NM_003883
• Gene symbol: HDAC3
• Gene ID (NCBI): 8841
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

What is the molecular weight of HDAC3? Is HDAC3 post-translationally modified?

The molecular weight of HDAC3 is 49 kDa but HDAC3 can form homodimers and homotrimers in cells (PMID: 11779848). HDAC3 can be phosphorylated at S424 residue by protein kinase CK2, which regulates HDAC3 activity (PMID: 15805470).

 What is the subcellular localization of HDAC3?

Unlike other class I HDACs, HDAC3 actively shuttles between the nucleus and cytoplasm (PMID: 11779848). Additionally, HDAC3 can be present at the plasma membrane, where it associates with c-Src (PMID: 16532030).

 What is the tissue expression pattern of HDAC3?

Like other class I HDACs, HDAC3 is ubiquitously expressed (PMID: 9346952).

 What is the mechanism of HDAC3 in regulating gene expression?

Unlike other histone deacetylates, it exists in a complex with two proteins - nuclear receptor co-repressor (N-CoR) and silencing mediator for retinoid and thyroid receptors (SMRT) - and therefore acts as a mediator of the repression function of unliganded nuclear hormone receptors (PMID: 10809664). N-CoR and SMRT play an active role in the recruitment of HDAC3 to binding sites as well as directly regulating HDAC3 activity. Additionally, HDAC3 targets growth-regulated genes.