HDAC2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

HDAC2 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1A3E4

Rezensionen (1)

Kat-Nr. : 67165-1-Ig

Synonyme

HD2, HDAC2, histone deacetylase 2, RPD3, YAF1

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Galerie der Validierungsdaten

• 

  WB analysis of MCF-7 using 67165-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67165-1-Ig (HDAC2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 67165-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67165-1-Ig (HDAC2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67165-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67165-1-Ig (HDAC2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67165-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67165-1-Ig (HDAC2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67165-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67165-1-Ig (HDAC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67165-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67165-1-Ig (HDAC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67165-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC2 antibody (67165-1-Ig, Clone: 1A3E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 67165-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HDAC2 (67165-1-Ig, Clone:1A3E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HepG2-Zellen, Jurkat-Zellen, NCCIT-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:400-1:1600
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

67165-1-Ig bindet in WB, IP, IHC, IF, FC, CoIP, ELISA HDAC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HDAC2 fusion protein Ag21288
• Vollständiger Name: histone deacetylase 2
• Berechnetes Molekulargewicht: 458 aa, 52 kDa; 488 aa,55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC031055
• Gene symbol: HDAC2
• Gene ID (NCBI): 3066
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is raised against residues near the C terminus of human HDAC2.

Publikationen

Species	Application	Title
human	WB,CoIP	iScience; Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression; Authors - Zhilei Zhang ; KD Validated
human	WB	Molecules; Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway; Authors - Xintian Lan
human,rat	WB,IF,IP	Toxicol Appl Pharmacol; Advanced oxidation protein products upregulate ABCB1 expression and activity via HDAC2-Foxo3α-mediated signaling in vitro and in vivo.; Authors - Tianrong Xun ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xiaoyu (Verified Customer) (06-28-2023)

Good for wb

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

HDAC2 Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:67165-1-Ig)

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011