HDAC11 Monoklonaler Antikörper

HDAC11 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1G12B5

Kat-Nr. : 67949-1-Ig

Synonyme

FLJ22237, HD11, HDAC11, histone deacetylase 11

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Galerie der Validierungsdaten

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  WB analysis using 67949-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67949-1-Ig (HDAC11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 67949-1-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67949-1-Ig (HDAC11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 67949-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67949-1-Ig (HDAC11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-231-Zellen, SKOV-3-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Kolonkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

67949-1-Ig bindet in WB, IHC, ELISA HDAC11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HDAC11 fusion protein Ag28335
• Vollständiger Name: histone deacetylase 11
• Berechnetes Molekulargewicht: 347 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC009676
• Gene symbol: HDAC11
• Gene ID (NCBI): 79885
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC11 belongs to Class IV HDACs. HDAC11 is localized to the nucleus and may be involved in regulating the expression of interleukin 10. Alternative splicing results in multiple transcript variants. HDAC11 has 2 isoforms with the molecular mass of 33 and 39 kDa.

Publikationen

Species	Application	Title
human	IHC	Transl Lung Cancer Res; Clinicopathological characteristics and prognostic significance of HDAC11 protein expression in non-small cell lung cancer: a retrospective study.; Authors - Di Lu
human	WB	Cell Discov; Lysine butyrylation of HSP90 regulated by KAT8 and HDAC11 confers chemoresistance; Authors - Yan He