HDAC10 Polyklonaler Antikörper

HDAC10 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 24913-1-AP

Synonyme

HD10, HDAC10, histone deacetylase 10

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Galerie der Validierungsdaten

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  WB analysis of fetal human brain using 24913-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 24913-1-AP (HDAC10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 24913-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 24913-1-AP (HDAC10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 24913-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24913-1-AP (HDAC10 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 24913-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24913-1-AP (HDAC10 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 24913-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24913-1-AP (HDAC10 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 24913-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24913-1-AP (HDAC10 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of MCF-7 using 24913-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 24913-1-AP (HDAC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

24913-1-AP bindet in WB, IHC, IF, ELISA HDAC10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HDAC10 fusion protein Ag18470
• Vollständiger Name: histone deacetylase 10
• Berechnetes Molekulargewicht: 669 aa, 71 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC125083
• Gene symbol: HDAC10
• Gene ID (NCBI): 83933
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HDAC10, also named as Histone deacetylase 10, is a 669 amino acid protein, which is widely expressed with high levels in liver and kidney. HDAC10, which belongs to the class IIb subfamily, has an active deacetylase domain (DAC) and a catalytically inactive leucine-rich domain (LRD). HDAC10 is determined that it plays a role in homologous recombination, promotes autophagy and survival in neuroblastoma cells, suppresses cervical cancer metastasis and facilitates the cell cycle.

Publikationen

Species	Application	Title
mouse, rat	WB	Kidney Int; Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.; Authors - Xiaojie Wang
human	IHC	Oncol Lett; HDAC10 expression is associated with DNA mismatch repair gene and is a predictor of good prognosis in colon carcinoma.; Authors - Xiangxiang Tao
human	WB	Cell Death Dis; WNT5A promotes the metastasis of esophageal squamous cell carcinoma by activating the HDAC7/SNAIL signaling pathway.; Authors - Yingtong Feng
human	IHC,WB	J Cell Mol Med; RNA m5C methylation orchestrates BLCA progression via macrophage reprogramming; Authors - Dali Yan