Orexin receptor 1 Polyklonaler Antikörper

Orexin receptor 1 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 18370-1-AP

Synonyme

HCRTR1, hypocretin (orexin) receptor 1, Hypocretin receptor type 1, Orexin receptor 1, Orexin receptor type 1, Ox 1 R, Ox1 R, OX1R

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 18370-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18370-1-AP (Orexin receptor 1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 18370-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18370-1-AP (Orexin receptor 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 18370-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18370-1-AP (Orexin receptor 1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18370-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18370-1-AP (Orexin receptor 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 18370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18370-1-AP (Orexin receptor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18370-1-AP (Orexin receptor 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18370-1-AP (Orexin receptor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 18370-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug Orexin receptor 1 antibody (18370-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Jurkat-Zellen, Maus-Pankreasgewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

18370-1-AP bindet in WB, IHC, IF, FC, ELISA Orexin receptor 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Orexin receptor 1 fusion protein Ag12269
• Vollständiger Name: hypocretin (orexin) receptor 1
• Berechnetes Molekulargewicht: 425 aa, 48 kDa
• Beobachtetes Molekulargewicht: 48-54 kDa
• GenBank-Zugangsnummer: BC074796
• Gene symbol: HCRTR1
• Gene ID (NCBI): 3061
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The hypocretin receptor 1 (OX1R, also named HCRTR1) is a G protein-coupled receptor and is a critical participant in the regulation of motivated behavior (PMID:28971565). HCRTR1 is associated with the regulation of motivation, reward, and autonomic functions. HCRTR1 shows a selective binding affinity for HCRT1/orexin-A. Moreover, HCRTR1 and HCRTR2 share 64% sequence similarity in humans (PMID:34052813).

Publikationen

Species	Application	Title
mouse	WB,IHC	Front Pharmacol; The Ciji-Hua'ai-Baosheng II Formula Attenuates Chemotherapy-Induced Anorexia in Mice With H22 Hepatocellular Carcinoma.; Authors - Shengyan Xi
mouse	WB	Brain Res Bull; Aerobic training associated with an active lifestyle exerts a protective effect against oxidative damage in hypothalamus and liver: The involvement of energy metabolism.; Authors - Pedro P M Scariot
mouse	WB	Drug Des Devel Ther; Orexin-A Reverse Bone Mass Loss Induced by Chronic Intermittent Hypoxia Through OX1R-Nrf2/HIF-1α Pathway.; Authors - Hong Gu
rat	WB	Evid Based Complement Alternat Med; Soporific Effect of Modified Suanzaoren Decoction and Its Effects on the Expression of CCK-8 and Orexin-A.; Authors - Liang-Hui Zhan
human	WB	Biochim Biophys Acta Mol Basis Dis; Orexin-A/OX1R is involved in regulation of autophagy to promote cortisol secretion in adrenocortical cell; Authors - Xin Guo
mouse	IF	Nat Biotechnol; Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells; Authors - Yunlong Tao