HCFC1 Polyklonaler Antikörper

HCFC1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 19358-1-AP

Synonyme

C1 factor, CFF, HCF, HCF 1, HCF1, HCFC1, HFC1, Host cell factor 1, VCAF, VP16 accessory protein

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 19358-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19358-1-AP (HCFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 19358-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19358-1-AP (HCFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19358-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19358-1-AP (HCFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 19358-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19358-1-AP (HCFC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 19358-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19358-1-AP (HCFC1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

19358-1-AP bindet in WB, IF, ELISA HCFC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HCFC1 fusion protein Ag6798
• Vollständiger Name: host cell factor C1 (VP16-accessory protein)
• Berechnetes Molekulargewicht: 209 kDa
• Beobachtetes Molekulargewicht: 230 kDa, 300 kDa
• GenBank-Zugangsnummer: BC063435
• Gene symbol: HCFC1
• Gene ID (NCBI): 3054
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Host cell factor 1 (HCFC1), also known as HCF-1, is a transcriptional co-regulator with a proteolytic cleavage at the PRO repeats that could be cleaved by O-GlcNAc transferase (OGT) to produce a heterodimeric complex of HCFC1N and HCFC1C subunits (PMID: 23539139). HCFC1 was first identified in herpes simplex virus transcription while it lacks a DNA binding domain. HCFC1 is not a traditional transcription factor, it interacts with multiple protein including transcription factors to regulate many biological processes, such as cell proliferation, migration, cell death and mitochondrial biogenesis (PMID: 25281006). Mutation in HCFC1 was related to an X-linked recessive disease ---cblX with a complex clinical manifestation involved in error of metabolism,  neurocognitive impairment and  craniofacial abnormalities (PMID: 24011988; PMID: 25281006).

Publikationen

Species	Application	Title
human,rat	IF	Microbiol Immunol; Susceptibility of rat immortalized neuronal cell line Rn33B expressing equine major histocompatibility class 1 to equine herpesvirus-1 infection is differentiation-dependent.; Authors - Erina Minato