Hemoglobin Alpha Polyklonaler Antikörper

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• KD/KO Validated

Hemoglobin Alpha Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 14537-1-AP

Synonyme

Alpha globin, HBA1, HBA2, Hemoglobin a, Hemoglobin Alpha, Hemoglobin alpha chain, Hemoglobin subunit alpha, hemoglobin, alpha 1

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Galerie der Validierungsdaten

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  WB analysis using 14537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14537-1-AP (Hemoglobin Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14537-1-AP (Hemoglobin Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14537-1-AP

  IP Result of anti-Hemoglobin alpha (IP:14537-1-AP, 4ug; Detection:14537-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IF Staining of human appendicitis using 14537-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Hemoglobin Alpha antibody (14537-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, Mausherzgewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IF: humanes Appendizitis-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14537-1-AP bindet in WB, IP, IHC, IF, ELISA Hemoglobin Alpha und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Hemoglobin Alpha fusion protein Ag6040
• Vollständiger Name: hemoglobin, alpha 1
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 14-16 kDa, 27 kDa
• GenBank-Zugangsnummer: BC050661
• Gene symbol: HBA1
• Gene ID (NCBI): 3039
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.

Publikationen

Species	Application	Title
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
mouse	WB,IHC	Int J Mol Sci; Regulation and Role of Neuron-Derived Hemoglobin in the Mouse Hippocampus.; Authors - Yujiao Lu ; KD Validated
human	WB	Stem Cell Reports; FAM122A Inhibits Erythroid Differentiation through GATA1.; Authors - Jing Chen
mouse	WB	Haematologica; Therapeutic effects of induced pluripotent stem cells in chimeric mice with beta-thalassemia.; Authors - Guanheng Yang
mouse	WB	Nutrients; Cellular Zinc Deficiency Impairs Heme Biosynthesis in Developing Erythroid Progenitors; Authors - Juyoung Kim
mouse		Arch Biochem Biophys; SOD1 deficiency decreases proteasomal function, leading to the accumulation of ubiquitinated proteins in erythrocytes.; Authors - Takujiro Homma