HARS Polyklonaler Antikörper

HARS Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 16375-1-AP

Synonyme

HARS, HisRS, Histidine tRNA ligase, histidyl tRNA synthetase, HRS

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 16375-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16375-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 16375-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16375-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16375-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16375-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16375-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 16375-1-AP

  Immunofluorescent analysis of MCF-7 cells, using HARS antibody 16375-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HeLa-Zellen, humanes Nierengewebe, K-562-Zellen, Mausherzgewebe, Maushirngewebe, Mauslungengewebe
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

16375-1-AP bindet in WB, IF, ELISA HARS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HARS fusion protein Ag9333
• Vollständiger Name: histidyl-tRNA synthetase
• Berechnetes Molekulargewicht: 509 aa, 57 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC011807
• Gene symbol: HARS
• Gene ID (NCBI): 3035
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HARS is a cytoplasmic enzyme that belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics.; Authors - Maria C Passarelli