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HA Tag Monoklonaler Antikörper

HA Tag Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

rekombinanten Protein

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F5C6

Kat-Nr. : CL488-66006

Synonyme

YPYDVPDYA

Galerie der Validierungsdaten

Filter:
  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using CoraLite® Plus 488-conjugated HA Tag Monoclonal an (CL488-66006)

    IF Staining of Transfected HEK-293 using CL488-66006

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 488 HA Tag antibody (CL488-66006, Clone: 1F5C6 ) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IFTransfizierte HEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IFSee 1 publications below

Produktinformation

CL488-66006 bindet in IF HA Tag und zeigt Reaktivität mit rekombinanten Protein

Getestete Reaktivität rekombinanten Protein
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name HA Tag
Gene symbol
Gene ID (NCBI)
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 HA Tag antibody CL488-66006Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
IF

Cell Rep

Hypoxia-sensitive cells trigger NK cell activation via the KLF4-ASH1L-ICAM-1 axis, contributing to impairment in the rat epididymis

Authors - Jitao Zeng
View Article