HA Tag Rekombinanter Antikörper

HA Tag Rekombinant Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein und mehr (1)

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

6I21

Kat-Nr. : 81290-1-RR

Synonyme

HA, HA Tag

---

Galerie der Validierungsdaten

• 

  WB analysis using 81290-1-RR

  HA-tag recombinant protein, non-transfected HEK-293 cells, transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 81290-1-RR (HA Tag antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Transfected HEK-293T using 81290-1-RR

  IP result of anti-HA Tag(IP:81290-1-RR, 4ug; Detection:81290-1-RR 1:20000) with Transfected HEK-293T cells lysate 795 ug.

• 

  IF Staining of Transfected HEK-293 using 81290-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA Tag antibody (81290-1-RR, Clone: 6I21 ) at dilution of 1:750 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Transfizierte HEK-293-Zellen
• Erfolgreiche IP: Transfected HEK-293T cells
• Erfolgreiche Detektion in IF: Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:375-1:1500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

81290-1-RR bindet in WB, IP, IF, ELISA HA Tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: HA Tag
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Publikationen

Species	Application	Title
mouse	IF	Nat Cell Biol; Manganese regulation of COPII condensation controls circulating lipid homeostasis; Authors - Xiao Wang
	WB	Mol Nutr Food Res; Ethanol Extract of Citrus grandis 'Tomentosa' Exerts Anticancer Effects by Targeting Skp2/p27 Pathway in Non-Small Cell Lung Cancer; Authors - Da Huang
		Res Sq; VprBP/DCAF1 triggers melanomagenic gene silencing through histone H2A phosphorylation; Authors - Yonghwan Shin
	WB	Int J Biol Sci; Hypoxia-driven TNS4 fosters HNSCC tumorigenesis by stabilizing integrin α5β1 complex and triggering FAK-mediated Akt and TGFβ signaling pathways; Authors - Xinyuan Zhao
mouse	WB	Front Cell Dev Biol; EVC-EVC2 complex stability and ciliary targeting are regulated by modification with ubiquitin and SUMO; Authors - Pablo Barbeito