H6PD Polyklonaler Antikörper

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• KD/KO Validated

H6PD Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15255-1-AP

Synonyme

6PGL, G6PDH, GDH, H6PD

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 15255-1-AP

  WB result of H6PD antibody (15255-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-H6PD transfected HepG2 cells.

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  WB analysis of HepG2 using 15255-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15255-1-AP (H6PD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15255-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15255-1-AP (H6PD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15255-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15255-1-AP (H6PD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15255-1-AP

  IP Result of anti-H6PD (IP:15255-1-AP, 4ug; Detection:15255-1-AP 1:1000) with HepG2 cells lysate 1600ug.

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  IHC staining of human hepatocirrhosis using 15255-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 15255-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 15255-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15255-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15255-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 15255-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, Mauslebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

15255-1-AP bindet in WB, IP, IHC, IF, ELISA H6PD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: H6PD fusion protein Ag7109
• Vollständiger Name: hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 89-95 kDa
• GenBank-Zugangsnummer: BC081559
• Gene symbol: H6PD
• Gene ID (NCBI): 9563
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hexose-6-phosphate dehydrogenase (H6PD) is also named as GDH,6PGL. It is the main NADPH generating enzyme in the lumen of the endoplasmic reticulum. H6PD is regarded as an ancillary enzyme in prereceptorial glucocorticoid activation and probably acts as a nutrient sensor and as a prosurvival factor(PMID:21620971). H6PD is a glycoprotein and has been shown tissue differences in glycosylation status might explain both the difference in migration on SDS-PAGE gels as well as in activity. Defects in H6PD are a cause of cortisone reductase deficiency (CRD). This antibody is specific to H6PD.

Publikationen

Species	Application	Title
rat	IF	Basic Res Cardiol; Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.; Authors - Alzbeta Hulikova
human,mouse	WB	iScience; A fingerprint of 2-[18F]FDG radiometabolites - How tissue-specific metabolism beyond 2-[18F]FDG-6-P could affect tracer accumulation; Authors - Eva-Maria Patronas ; KD Validated
	WB	Biomolecules; PMID: 31480692; Authors - Xixi Guo