H2AFY2 Polyklonaler Antikörper

H2AFY2 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 17030-1-AP

Synonyme

Core histone macro H2A.2, H2A histone family, member Y2, H2AFY2, histone, Histone macroH2A2, macroH2A2, mH2A2

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 17030-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17030-1-AP (H2AFY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17030-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17030-1-AP (H2AFY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 17030-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17030-1-AP (H2AFY2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17030-1-AP

  IP Result of anti-H2AFY2 (IP:17030-1-AP, 4ug; Detection:17030-1-AP 1:500) with HepG2 cells lysate 1000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Lebergewebe, Mausnierengewebe
• Erfolgreiche IP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

17030-1-AP bindet in WB, IP, ELISA H2AFY2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: H2AFY2 fusion protein Ag10487
• Vollständiger Name: H2A histone family, member Y2
• Berechnetes Molekulargewicht: 372 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC016172
• Gene symbol: H2AFY2
• Gene ID (NCBI): 55506
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	J Cell Physiol; MCPIP1 regulates RORα expression to protect against liver injury induced by lipopolysaccharide via modulation of miR-155.; Authors - Zhenzhen Li
mouse	WB	Cell Physiol Biochem; Klf4 Alleviates Lipopolysaccharide-Induced Inflammation by Inducing Expression of MCP-1 Induced Protein 1 to Deubiquitinate TRAF6.; Authors - Zhenzhen Li
mouse	WB	Immunol Cell Biol; ING4 alleviated lipopolysaccharide-induced inflammation by regulating the NF-κB pathway via a direct interaction with SIRT1.; Authors - Yunshu Yang
mouse	WB	Arch Biochem Biophys; ROR alpha protects against LPS-induced inflammation by down-regulating SIRT1/NF-kappa B pathway.; Authors - Shichao Han
mouse	WB	J Cell Physiol; MCPIP1 alleviated lipopolysaccharide-induced liver injury by regulating SIRT1 via modulation of microRNA-9.; Authors - Shichao Han