Glutamine Synthetase Monoklonaler Antikörper

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• KD/KO Validated

Glutamine Synthetase Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B6G4

Kat-Nr. : 66323-2-Ig

Synonyme

GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 66323-2-Ig

  WB result of Glutamine synthetase antibody (66323-2-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells.

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  IHC staining of human liver cancer using 66323-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66323-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66323-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 66323-2-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 66323-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 66323-2-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 66323-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66323-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 66323-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Glutamine Synthetase antibody (66323-2-Ig, Clone: 1B6G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 66323-2-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Glutamine Synthetase (66323-2-Ig, Clone:1B6G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Mauslebergewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mauslebergewebe
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:2500-1:10000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66323-2-Ig bindet in WB, IHC, IF, FC, ELISA Glutamine Synthetase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Glutamine Synthetase fusion protein Ag6309
• Vollständiger Name: glutamate-ammonia ligase (glutamine synthetase)
• Berechnetes Molekulargewicht: 374 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC011700
• Gene symbol: GLUL
• Gene ID (NCBI): 2752
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.