Glucagon Monoklonaler Antikörper

Glucagon Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen

1

CloneNo.

2A8G1

Kat-Nr. : CL488-67286

Synonyme

GCG, GLP 2, GLP1, GLP2, glucagon, GRPP, GRPP Oxyntomodulin, OXM

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Galerie der Validierungsdaten

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  IF Staining of mouse pancreas using CL488-67286

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using the CoraLite® Plus 488-conjugated version of this antibody, CL488-67286 (Glucagon antibody) and CL594-66198 (INS antibody), at dilution of 1:400. The B cells of the islets of Langerhans was labelled in red with CL594-66198(INS antibody).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maus-Pankreasgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL488-67286 bindet in IF Glucagon und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Glucagon fusion protein Ag10629
• Vollständiger Name: glucagon
• Berechnetes Molekulargewicht: 180 aa, 21 kDa
• GenBank-Zugangsnummer: BC005278
• Gene symbol: GCG
• Gene ID (NCBI): 2641
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glucagon is a 29-amino acid peptide hormone secreted from the pancreatic alpha cells with a powerful stimulatory effect on hepatic glucose production acting to increase plasma glucose levels. Glucagon is best known as the counter-regulatory hormone to INS, and normal glucose homeostasis depends largely on the balanced secretion of INS and glucagon fromthe pancreatic beta and alpha cells, respectively.The regulation of glucose metabolism by glucagon is mediated by its direct action on the peripheral tissues such as the liver and also by the brain. Glucagon is also released postprandially in a transient manner, which was shown to be involved in inhibition of food intake via reduction of meal size.

Publikationen

Species	Application	Title
mouse	IF	iScience; Optimal HSF1 activation in response to acute cold stress in BAT requires nuclear TXNIP; Authors - Althea N Waldhart