Giantin Polyklonaler Antikörper

Giantin Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 22270-1-AP

Synonyme

Cell and organelle markers, GCP, GCP372, giantin, GOLGB1, Golgi marker, Golgin subfamily B member 1, GOLIM1, Macrogolgin

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Galerie der Validierungsdaten

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  WB analysis using 22270-1-AP

  Various lysates were subjected to Tris-Acetate gel system followed by western blot with 22270-1-AP (Giantin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 22270-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Giantin antibody (22270-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 22270-1-AP

  Immunofluorescent analysis of HepG2 cells using 22270-1-AP (Giantin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 22270-1-AP

  Immunofluorescent analysis of HeLa cells using 22270-1-AP (Giantin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 4 publications below

Produktinformation

22270-1-AP bindet in WB, IF, ELISA Giantin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: golgin B1, golgi integral membrane protein
• Berechnetes Molekulargewicht: 376 kDa
• Beobachtetes Molekulargewicht: 376 kDa
• GenBank-Zugangsnummer: NM_004487
• Gene symbol: GOLGB1
• Gene ID (NCBI): 2804
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Giantin is a conserved Golgi membrane protein containing a large cytoplasmic domain. It may function to form the intercisternal cross-bridges of the Golgi complex. Anti-Giantin has been widely used to label the Golgi apparatus.

Publikationen

Species	Application	Title
human	IF	iScience; Cytosolic Ca2+ Modulates Golgi Structure Through PKCα-Mediated GRASP55 Phosphorylation.; Authors - Stephen Ireland
human		Int J Nanomedicine; PMID: 28769563; Authors - Huiying Xue
human	IF	J Alzheimers Dis; High Fat Diet Mediates Amyloid-β Cleaving Enzyme 1 Phosphorylation and SUMOylation, Enhancing Cognitive Impairment in APP/PS1 Mice; Authors - Jian Bao
human	WB	Methods Mol Biol; Immunofluorescence Microscopy of the Mammalian Golgi Apparatus; Authors - Maryam Arab
monkey	IF	Seizure; Heterozygous RELN missense variants associated with genetic generalized epilepsy; Authors - Xiaoling Wu
human	IF	Nat Methods; StayGold variants for molecular fusion and membrane-targeting applications; Authors - Ryoko Ando

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (04-28-2023)

Detected a band around the expected size, however the signal was really weak.

• Applications: Western Blot