Geminin Polyklonaler Antikörper

Geminin Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-10802

Synonyme

Gem, Geminin, GMNN, RP3 369A17.3

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using CL488-10802

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Geminin antibody (CL488-10802) at dilution of 1:200.

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  FC experiment of MCF-7 using CL488-10802

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human Geminin (CL488-10802) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of MCF-7 using CL488-10802

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human Geminin (CL488-10802) (right), or 0.2 ug Control Antibody (left). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-10802 bindet in IF, FC (Intra) Geminin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Geminin fusion protein Ag1076
• Vollständiger Name: geminin, DNA replication inhibitor
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 28-29 kDa
• GenBank-Zugangsnummer: BC005185
• Gene symbol: GMNN
• Gene ID (NCBI): 51053
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011