Gelsolin Monoklonaler Antikörper

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• KD/KO Validated

Gelsolin Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgA

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3C4A2

Kat-Nr. : 66280-1-Ig

Synonyme

Actin depolymerizing factor, ADF, AGEL, Brevin, Gelsolin, GSN

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Galerie der Validierungsdaten

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  WB analysis of U2OS using 66280-1-Ig

  U2OS (WT and GSN KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 66280-1-Ig incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of human plasma using 66280-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 66280-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66280-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 66280-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 66280-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 66280-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66280-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe, A549-Zellen, HT-29-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66280-1-Ig bindet in WB, IHC, ELISA Gelsolin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgA
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gelsolin fusion protein Ag17916
• Vollständiger Name: gelsolin (amyloidosis, Finnish type)
• Berechnetes Molekulargewicht: 782 aa, 86 kDa
• Beobachtetes Molekulargewicht: 80-83 kDa
• GenBank-Zugangsnummer: BC026033
• Gene symbol: GSN
• Gene ID (NCBI): 2934
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Thiophiler Affinitätschromatograph
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease.