Galectin-1 Monoklonaler Antikörper

Galectin-1 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen

1

CloneNo.

3G10D2

Rezensionen (1)

Kat-Nr. : CL488-60223

Synonyme

14 kDa laminin binding protein, 14 kDa lectin, DKFZp686E23103, Gal 1, GAL1, Galaptin, Galectin 1, Galectin-1, GBP, HBL, HLBP14, HPL, Lactose binding lectin 1, LGALS1, S Lac lectin 1

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Galerie der Validierungsdaten

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  IF Staining of human colon cancer using CL488-60223

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 Galectin-1 antibody (CL488-60223, Clone: 3G10D2 ) at dilution of 1:800.

• 

  IF Staining of U2OS using CL488-60223

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 Galectin-1 antibody (CL488-60223, Clone: 3G10D2 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Kolonkarzinomgewebe, U2OS-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL488-60223 bindet in IF Galectin-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Galectin-1 fusion protein Ag2432
• Vollständiger Name: lectin, galactoside-binding, soluble, 1
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC020675
• Gene symbol: LGALS1
• Gene ID (NCBI): 3956
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

Publikationen

Species	Application	Title
human	IF	Sci Rep; The role of GCNT1 mediated O-glycosylation in aggressive prostate cancer; Authors - Kirsty Hodgson

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Margarita (Verified Customer) (06-06-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: PC3 cells

CL Plus 488 Galectin-1 Antibody Immunofluorescence validation (1:200 dilution) in PC3 cells (Cat no:CL488-60223)