Granzyme K Monoklonaler Antikörper

Granzyme K Monoklonal Antikörper für FC, IF, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1C3A4

Kat-Nr. : 67272-1-Ig

Synonyme

Fragmentin 3, Granzyme 3, Granzyme K, GZMK, NK Tryp 2, NK tryptase 2, TRYP2

---

Galerie der Validierungsdaten

• 

  IHC staining of human tonsillitis using 67272-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67272-1-Ig (GZMK antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67272-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67272-1-Ig (GZMK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67272-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67272-1-Ig (GZMK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 67272-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Granzyme K antibody (67272-1-Ig, Clone: 1C3A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

• 

  FC experiment of NIH/3T3 using 67272-1-Ig

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human Granzyme K (67272-1-Ig, Clone:1C3A4) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

67272-1-Ig bindet in IHC, IF, FC, ELISA Granzyme K und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Granzyme K fusion protein Ag27297
• Vollständiger Name: granzyme K (granzyme 3; NK tryptase II)
• Berechnetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC035802
• Gene symbol: GZMK
• Gene ID (NCBI): 3003
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Granzymes are a family of serine proteases stored in granules inside cytotoxic cells of the immune system. There are five human granzymes (granzyme A (GrA), GrB, GrH, GrK and GrM) currently identified, whereas mice have ten known granzymes (GrA-G, GrK, GrM and GrN) (PMID:14499263). Human GrK was first discovered in 1988 after purification from human peripheral blood mononuclear cells. GrK is expressed by cytotoxic T lymphocytes, natural killer T cells (NKT), γδ T cells and CD56bright+ NK cells.

Publikationen

Species	Application	Title
human	IHC	Biomedicines; Identification and Preliminary Clinical Validation of Key Extracellular Proteins as the Potential Biomarkers in Hashimoto's Thyroiditis by Comprehensive Analysis; Authors - Zihan Xi
human	IF	Adv Sci (Weinh); Behind the Indolent Facade: Uncovering the Molecular Features and Malignancy Potential in Lung Minimally Invasive Adenocarcinoma by Single-Cell Transcriptomics; Authors - Xin Zhang