GUCY1B3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GUCY1B3 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 19011-1-AP

Synonyme

GC S beta 1, GC SB3, GCS beta 1, GCS beta 3, GUC1B3, GUCB3, GUCSB3, GUCY1B1, GUCY1B3

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Galerie der Validierungsdaten

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  WB analysis using 19011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19011-1-AP (GUCY1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 19011-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19011-1-AP (GUCY1B3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 19011-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19011-1-AP (GUCY1B3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 19011-1-AP

  IP result of anti-GUCY1B3 (IP:19011-1-AP, 4ug; Detection:19011-1-AP 1:500) with human placenta tissue lysate 2600 ug.

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  IHC staining of mouse brain using 19011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19011-1-AP (GUCY1B3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19011-1-AP (GUCY1B3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 19011-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19011-1-AP (GUCY1B3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19011-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19011-1-AP (GUCY1B3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 19011-1-AP

  Immunofluorescent analysis of Hela cells, using GUCY1B3 antibody 19011-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of MCF-7 using 19011-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GUCY1B3 antibody 19011-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, Mauslungengewebe, Rattenlungengewebe
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Produktinformation

19011-1-AP bindet in WB, IP, IHC, IF, ELISA GUCY1B3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GUCY1B3 fusion protein Ag5196
• Vollständiger Name: guanylate cyclase 1, soluble, beta 3
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 67-71 kDa
• GenBank-Zugangsnummer: BC047620
• Gene symbol: GUCY1B3
• Gene ID (NCBI): 2983
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Aortic disease in Marfan syndrome is caused by overactivation of sGC-PRKG signaling by NO.; Authors - Andrea de la Fuente-Alonso
mouse	WB,IHC	Clin Sci (Lond); Empagliflozin prevents cardiomyopathy via sGC-cGMP-PKG pathway in type 2 diabetes mice.; Authors - Mei Xue ; KD Validated
mouse	WB,IHC	Sci Rep; Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway.; Authors - Catarina Rippe
mouse	WB	Cell Tissue Res; Cavin-3 (PRKCDBP) deficiency reduces the density of caveolae in smooth muscle.; Authors - Baoyi Zhu
rat	WB	World Neurosurg; Upregulation of Connexin 40 Mediated by Nitric Oxide Attenuates Cerebral Vasospasm After Subarachnoid Hemorrhage via the Nitric Oxide-Cyclic Guanosine Monophosphate-Protein Kinase G Pathway.; Authors - Shi Hai Lan
human	WB	Front Endocrinol (Lausanne); Specific dilation pattern in placental circulation and the NO/sGC role in preeclampsia placental vessels; Authors - Jiaqi Tang