GTSE1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GTSE1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 21319-1-AP

Synonyme

B99, G 2 and S phase expressed 1, GTSE 1, GTSE1, Protein B99 homolog

---

Galerie der Validierungsdaten

• 

  WB analysis using 21319-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21319-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21319-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21319-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 21319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 21319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, L02-Zellen, Mauslebergewebe, MCF-7-Zellen, Rattenlebergewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Produktinformation

21319-1-AP bindet in WB, IHC, IF, ELISA GTSE1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GTSE1 fusion protein Ag13497
• Vollständiger Name: G-2 and S-phase expressed 1
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 66-77 kDa, 100 kDa
• GenBank-Zugangsnummer: BC006325
• Gene symbol: GTSE1
• Gene ID (NCBI): 51512
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GTSE1 is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis.

Publikationen

Species	Application	Title
human	IHC	Mod Pathol; Expression profiling of small intestinal neuroendocrine tumors identifies subgroups with clinical relevance, prognostic markers and therapeutic targets.; Authors - Ellinor Andersson
human	WB	Mol Ther Nucleic Acids; lncRNA LINC00665 Stabilized by TAF15 Impeded the Malignant Biological Behaviors of Glioma Cells via STAU1-Mediated mRNA Degradation.; Authors - Xuelei Ruan
human	IHC	Cancer Sci; High G2 and S-phase expressed 1 expression promotes acral melanoma progression and correlates with poor clinical prognosis.; Authors - Tianxiao Xu
human,mouse	WB,IHC	Lab Invest; GTSE1 promotes prostate cancer cell proliferation via the SP1/FOXM1 signaling pathway.; Authors - Wenjie Lai ; KD Validated
human	WB,IHC	Lab Invest; GTSE1 promotes tumor growth and metastasis by attenuating of KLF4 expression in clear cell renal cell carcinoma.; Authors - Weihao Chen ; KD Validated
human	WB,IHC,IF	Mol Carcinog; G2 and S phase-expressed-1 induces chromosomal instability in esophageal squamous cell carcinoma cells and inhibits cell apoptosis through ROS/JNK signaling; Authors - Man Zhang ; KD Validated