GTF2IRD1 Polyklonaler Antikörper

GTF2IRD1 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17052-1-AP

Synonyme

BEN, CREAM1, GTF2IRD1, GTF3, hMusTRD1alpha1, MUSTRD1, MusTRD1/BEN, RBAP2, USE B1 binding protein, WBS, WBSCR11, WBSCR12

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 17052-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17052-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17052-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17052-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 17052-1-AP

  IP Result of anti-GTF2IRD1 (IP:17052-1-AP, 4ug; Detection:17052-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human testis using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 17052-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe, Mauslebergewebe, Mausnierengewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hirngewebe, humanes Lebergewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

17052-1-AP bindet in WB, IP, IHC, IF,ELISA GTF2IRD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GTF2IRD1 fusion protein Ag10718
• Vollständiger Name: GTF2I repeat domain containing 1
• Berechnetes Molekulargewicht: 959 aa, 106 kDa
• Beobachtetes Molekulargewicht: 145 kDa, 120 kDa
• GenBank-Zugangsnummer: BC018136
• Gene symbol: GTF2IRD1
• Gene ID (NCBI): 9569
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.