GTF2H2 Polyklonaler Antikörper

GTF2H2 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 16005-1-AP

Synonyme

BTF2, BTF2 p44, BTF2P44, GTF2H2, T BTF2P44, TFIIH

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 16005-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16005-1-AP (GTF2H2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16005-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16005-1-AP (GTF2H2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16005-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16005-1-AP (GTF2H2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16005-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16005-1-AP (GTF2H2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

16005-1-AP bindet in WB, ELISA GTF2H2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GTF2H2 fusion protein Ag8653
• Vollständiger Name: general transcription factor IIH, polypeptide 2, 44kDa
• Berechnetes Molekulargewicht: 395aa,44 kDa; 165aa,19 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC005345
• Gene symbol: GTF2H2
• Gene ID (NCBI): 2966
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transcription factor IIH(TFIIH) is associated with the RNA polymerase II transcription complex, which is involved in transcription and transcription-mediated DNA repair. GTF2H2(GENERAL TRANSCRIPTION FACTOR IIH, POLYPEPTIDE 2) is one of the six subnuits forming the core-TFIIH basal transcriptional factor. TFIIH plays a role in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N terminus of GTF2H2 interacts with and regulates XPD, and an intact C terminus is required for a successful escape of RNAPol II from the promoter. This is a rabbit polyclonal antibody producted by part of N-terminal GTF2H2 of human origin.

Publikationen

Species	Application	Title
human	WB	Cell; CLP1 Founder Mutation Links tRNA Splicing and Maturation to Cerebellar Development and Neurodegeneration.; Authors - Ashleigh E Schaffer
human	WB	Tissue Cell; Exosome-derived GTF2H2 from Huh7 cells can inhibit endothelial cell viability, migration, tube formation, and permeability; Authors - Zhenkun Li