GTF2F2 Polyklonaler Antikörper

GTF2F2 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15139-1-AP

Synonyme

ATP dependent helicase GTF2F2, BTF4, GTF2F2, RAP30, TF2F2, TFIIF, TFIIF beta

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 15139-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 15139-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 15139-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15139-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15139-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 15139-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15139-1-AP (GTF2F2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, Mausmilzgewebe, PC-3-Zellen, Rattenmilzgewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15139-1-AP bindet in WB, IF,ELISA GTF2F2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GTF2F2 fusion protein Ag7313
• Vollständiger Name: general transcription factor IIF, polypeptide 2, 30kDa
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC001771
• Gene symbol: GTF2F2
• Gene ID (NCBI): 2963
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.