GTF2B Polyklonaler Antikörper

GTF2B Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 16467-1-AP

Synonyme

GTF2B, S300 II, TF2B, TFIIB

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 16467-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16467-1-AP (GTF2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16467-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16467-1-AP (GTF2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16467-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16467-1-AP (GTF2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16467-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16467-1-AP (GTF2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 16467-1-AP

  IP Result of anti-GTF2B (IP:16467-1-AP, 4ug; Detection:16467-1-AP 1:300) with K-562 cells lysate 3200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche IP: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

16467-1-AP bindet in WB, IP, ELISA GTF2B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GTF2B fusion protein Ag9547
• Vollständiger Name: general transcription factor IIB
• Berechnetes Molekulargewicht: 316 aa, 35 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC020597
• Gene symbol: GTF2B
• Gene ID (NCBI): 2959
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	PLoS Biol; A novel TOX3-WDR5-ABCG2 signaling axis regulates the progression of colorectal cancer by accelerating stem-like traits and chemoresistance; Authors - Jiaojiao Hao
		Am J Cancer Res; PMID: 28123844; Authors - Jiaojiao Hao
human	WB	J Exp Clin Cancer Res; Melatonin synergizes BRAF-targeting agent vemurafenib in melanoma treatment by inhibiting iNOS/hTERT signaling and cancer-stem cell traits.; Authors - Jiaojiao Hao