GSTO1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GSTO1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 15124-1-AP

Synonyme

GSTO 1, GSTO1, GSTTLp28, P28

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Galerie der Validierungsdaten

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  WB analysis using 15124-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15124-1-AP (GSTO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 15124-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15124-1-AP (GSTO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15124-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15124-1-AP (GSTO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 15124-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 15124-1-AP (GSTO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15124-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15124-1-AP (GSTO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15124-1-AP

  IP Result of anti-GSTO1 (IP:15124-1-AP, 4ug; Detection:15124-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IHC staining of human pancreas cancer using 15124-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15124-1-AP (GSTO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15124-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15124-1-AP (GSTO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 15124-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GSTO1 antibody (15124-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Herzgewebe, Jurkat-Zellen, Mauslebergewebe, PC-3-Zellen, Rattenhirngewebe, U-937-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IF: See 2 publications below

Produktinformation

15124-1-AP bindet in WB, IP, IHC, IF, ELISA GSTO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSTO1 fusion protein Ag7199
• Vollständiger Name: glutathione S-transferase omega 1
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 27-30 kDa
• GenBank-Zugangsnummer: BC000127
• Gene symbol: GSTO1
• Gene ID (NCBI): 9446
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glutathione-S-transferase omega 1 (GSTO1) belongs to a new subfamily of GSTs and is the rate limiting enzyme for biotransformation of inorganic arsenic, environmental carcinogen. Expression of GSTO1-1 was abundant in a wide range of normal tissues, particularly liver, macrophages, glial cells, and endocrine cells. Human GSTO1 assembles as a dimer. This antibody detects both monomer (27-30 kDa) and dimer forms of GSTO1 (54-64 kDa). (PMID: 21495794)

Publikationen

Species	Application	Title
human	WB	Mol Cell; SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation; Authors - Wenxin Zhang
mouse	WB,IF	J Exp Med; ASC deglutathionylation is a checkpoint for NLRP3 inflammasome activation.; Authors - Shuhang Li ; KO Validated
pig	WB	Food Chem; TMT-based quantitative proteomic analysis of porcine muscle associated with postmortem meat quality.; Authors - Xinhua Hou
		Biochem J; Selenophosphate Synthetase 1 is an Essential Protein with Roles in Regulation of Redox Homeostasis in Mammals.; Authors - Ryuta Tobe
mouse	WB	Toxicol Appl Pharmacol; PMID: 24998975; Authors - Jing-Jing Chuang
human	WB	J Proteomics; Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.; Authors - Dan Zhang