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GST Tag Rekombinanter Antikörper

GST Tag Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein und mehr (1)

Anwendung

WB, CoIP, ELISA

Konjugation

Unkonjugiert

CloneNo.

9B24

Kat-Nr. : 80006-1-RR

Synonyme

Glutathione S-Transferase, GST, GST Tag

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of various lysates using GST Tag Recombinant antibody (80006-1-RR)

    WB analysis using 80006-1-RR

    GST tagged protein and other negative samples were subjected to SDS PAGE followed by western blot with 80006-1-RR (GST Tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBRecombinant protein

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

CoIPSee 1 publications below

Produktinformation

80006-1-RR bindet in WB, CoIP, ELISA GST Tag und zeigt Reaktivität mit rekombinanten Protein

Getestete Reaktivität rekombinanten Protein
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen GST Tag fusion protein Ag0040
Vollständiger Name GST Tag
Berechnetes Molekulargewicht 26 kDa
GenBank-ZugangsnummerFN315687
Gene symbol
Gene ID (NCBI)
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20℃ lagern. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody is a Rabbit recombinant antibody to GST tag.

Protokolle

Produktspezifische Protokolle
WB protocol for GST Tag antibody 80006-1-RRProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanCoIP

Vet Microbiol

PEDV N protein capture protein translation element PABPC1 and eIF4F to promote viral replication

Authors - Huanjie Zhai
View Article