GST Tag Monoklonaler Antikörper

GST Tag Monoklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

rekombinanten Protein, schistosoma japonicum und mehr (1)

Anwendung

WB, IP, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

91

CloneNo.

3G12B10

Rezensionen (3)

Kat-Nr. : 66001-2-Ig

Synonyme

GST, GST Tag

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• Galerie der Validierungsdaten
• Proteintech vs. Other Vendors

• 

  WB analysis of Recombinant protein using 66001-2-Ig

  GST tagged recombinant protein (25ng/lane) was subjected to SDS-PAGE followed by western blot with anti-GST tag monoclonal antibody 66001-2-Ig at various dilutions.

• 

  WB analysis of Recombinant protein using 66001-2-Ig

  GST protein (25 ng), pGEX6P-1 vector expressed in E. coli, were subjected to SDS PAGE followed by western blot with 66001-2-Ig (GST Tag antibody, 1:200,000) incubated at room temperature for 1.5 hours.

• 

  IP experiment of Recombinant protein using 66001-2-Ig

  IP result of anti-GST Tag(IP:66001-2-Ig, 5ug; Detection:66001-2-Ig 1:10000) with Total E. coli lysate (with pGEX4T fused plasmid) 1360 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein
• Erfolgreiche IP: E. coli-Lysat

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 75 publications below
• IF: See 4 publications below
• IP: See 15 publications below
• CoIP: See 3 publications below

Produktinformation

66001-2-Ig bindet in WB, IP, IF, CoIP, ELISA GST Tag und zeigt Reaktivität mit rekombinanten Protein, schistosoma japonicum

• Getestete Reaktivität: rekombinanten Protein, schistosoma japonicum
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: GST Tag
• Berechnetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: FN315687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the N- or C-terminus of targeted proteins.

Publikationen

Species	Application	Title
human	WB	Nat Cancer; METTL16 antagonizes MRE11-mediated DNA end resection and confers synthetic lethality to PARP inhibition in pancreatic ductal adenocarcinoma; Authors - Xiangyu Zeng
	WB	Nat Cell Biol; Phagolysosome resolution requires contacts with the endoplasmic reticulum and phosphatidylinositol-4-phosphate signalling.; Authors - Roni Levin-Konigsberg
	WB	Sci Bull (Beijing); Targeted clearance of mitochondria by an autophagy-tethering compound (ATTEC) and its potential therapeutic effects; Authors - Shuixia Tan
	WB	Nat Commun; Fungal secondary metabolism is governed by an RNA-binding protein CsdA/RsdA complex; Authors - Zili Song
	WB	Nat Commun; N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization; Authors - Weiji Weng
	WB,IP	Adv Sci (Weinh); Elevated Nuclear PHGDH Synergistically Functions with cMyc to Reshape the Immune Microenvironment of Liver Cancer; Authors - Hongwen Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lana (Verified Customer) (05-27-2022)

SDS-PAGE: 4-12% Bis-Tris gradient gel. Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C. Blocking: SEA Block Blocking Buffer 1h, room T. Primary Ab: O/N incubation at 4C, 1:1000. Secondary Ab: IRDye 680LT Goat anti-Mouse, 1:15000. Lines of WB image: 1 – protein ladder, 2 and 3 – GST protein different amounts.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Lysate of Sepharose beads after GST-pull down

GST Tag Antibody Western Blot validation (1:5000 dilution) in Lysate of Sepharose beads after GST-pull down (Cat no:66001-2-Ig)

FH

Susanne (Verified Customer) (11-18-2021)

Clear Signal, no background on E.coli lysate with expressed GST blotted on PVDF membrane

• Applications: Western Blot, Cell culture
• Primary Antibody Dilution: 1:30000
• Cell Tissue Type: E.coli lysate

FH

Aurelie (Verified Customer) (12-11-2018)

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1µl for IP and 1/5000 for WB
• Cell Tissue Type: in vitro

GST Tag Antibody Western Blot,Immunoprecipitation, validation (1µl for IP and 1/5000 for WB dilution) in in vitro (Cat no:66001-2-Ig)