GSR Polyklonaler Antikörper

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• KD/KO Validated

GSR Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 18257-1-AP

Synonyme

GLUR, glutathione reductase, GR, GRase, GRD1, GSR

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 18257-1-AP

  WB result of GSR antibody (18257-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSR transfected HeLa cells.

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  WB analysis using 18257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18257-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 18257-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 18257-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18257-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18257-1-AP

  IP Result of anti-GSR (IP:18257-1-AP, 4ug; Detection:18257-1-AP 1:500) with HeLa cells lysate 2600ug.

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  IHC staining of human breast cancer using 18257-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18257-1-AP (GSR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18257-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18257-1-AP (GSR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 18257-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSR antibody (18257-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, Maushodengewebe, Mauslungengewebe, Rattenhodengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 18 publications below

Produktinformation

18257-1-AP bindet in WB, IP, IHC, IF, ELISA GSR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSR fusion protein Ag13080
• Vollständiger Name: glutathione reductase
• Berechnetes Molekulargewicht: 522 aa, 56 kDa
• Beobachtetes Molekulargewicht: 52-55 kDa
• GenBank-Zugangsnummer: BC069244
• Gene symbol: GSR
• Gene ID (NCBI): 2936
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GSR(Glutathione reductase) is also named as GLUR, GRD1 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.It was first found in a black American a variant red cell GSR characterized by greater electrophoretic mobility and enzyme activity per unit of hemoglobin than the normal.This protein maintains high levels of reduced glutathione in the cytosol.It has 5 isoforms produced by alternative splicing and alternative initiation.It can also exsit as a homodimer with the molecular weight of 110KDa(PMID:8631352).This antibody is specific to GSR.

Publikationen

Species	Application	Title
human	WB	Redox Biol; Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation.; Authors - Angel Gallego-Selles
mouse	WB	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H
human	WB	Nutrients; A Mango Leaf Extract (Zynamite®) Combined with Quercetin Has Exercise-Mimetic Properties in Human Skeletal Muscle; Authors - Miriam Martinez-Canton
human	WB	Pharmacol Res; Phyto-sesquiterpene lactones DET and DETD-35 induce ferroptosis in vemurafenib sensitive and resistant melanoma via GPX4 inhibition and metabolic reprogramming.; Authors - Meng-Ting Chang
human	WB	Free Radic Biol Med; Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.; Authors - Kelei Dong
mouse	WB	Free Radic Biol Med; Antioxidant defense in quiescent cells determines selectivity of electron transport chain inhibition-induced cell death.; Authors - Jan Blecha