eRF3a/GSPT1 Monoklonaler Antikörper

eRF3a/GSPT1 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A3H6

Kat-Nr. : 68217-1-Ig

Synonyme

551G9.2, eRF3a, ETF3A, FLJ38048, FLJ39067, G1 to S phase transition 1, GSPT1, GST1

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Galerie der Validierungsdaten

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  WB analysis using 68217-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68217-1-Ig (eRF3a/GSPT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of COLO 320 using 68217-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 68217-1-Ig (GSPT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 68217-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68217-1-Ig (GSPT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 68217-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using eRF3a/GSPT1 antibody (68217-1-Ig, Clone: 2A3H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 68217-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using eRF3a/GSPT1 antibody (68217-1-Ig, Clone: 2A3H6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, COLO 320-Zellen, Hausschwein-Hirngewebe, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68217-1-Ig bindet in WB, IF, ELISA eRF3a/GSPT1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: eRF3a/GSPT1 fusion protein Ag27986
• Vollständiger Name: G1 to S phase transition 1
• Berechnetes Molekulargewicht: 68 aa, 4 kDa
• Beobachtetes Molekulargewicht: 80-85 kDa
• GenBank-Zugangsnummer: BC009503
• Gene symbol: GSPT1
• Gene ID (NCBI): 2935
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The eukaryotic Release Factor 3 (eRF3) is a GTPase that associates with eRF1 in a complex that mediates translation termination. Eukaryotic release factor 3 (eRF3) has many functions in eukaryotic cells, such as controlling the regulation of the cell cycle at the G1 to S phase transition, and regulating protein synthesis as a GTP dependent stimulator of eRF1 in translation termination. It was also reported to play a key role as an initiator of the mRNA degradation machinery in the recycling of ribosomes in successive cycles of translation,and probably also in transcription regulation. eRF3a, also known as GSPT1, is one subunit of eRF3(PMID:15917414,12923185). It involves in translation termination in response to the termination codons UAA, UAG and UGA and stimulates the activity of ERF1. eRF3a/GSPT1 exists some isoforms with MV 69 kDa and 56 kDa. Identification of a processed form of eRF3a/GSPT1 as a BIR3-binding protein-Using a GST-BIR3 fusion protein as an affinity reagent to purify new IAP binding proteins from extracts of human cells and mouse tissues, we previously isolated 3 proteins of molecular weights 23, 38 and 80 kDa. 80 kDa band confirmed that it is a processed form of the human GSPT1/eRF3a protein, lacking the first 69 residues(PMID: 12865429).