GSK3B Monoklonaler Antikörper

GSK3B Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human und mehr (2)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

2E9E4

Kat-Nr. : 67329-1-Ig

Synonyme

GSK 3 beta, GSK3A B, GSK3B, GSK3B 216P, gsk3beta, GSK3β

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Galerie der Validierungsdaten

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  WB analysis using 67329-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67329-1-Ig (GSK3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of pig brain using 67329-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67329-1-Ig (GSK3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67329-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67329-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67329-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67329-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67329-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSK3B antibody (67329-1-Ig, Clone: 2E9E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Hausschwein-Hirngewebe, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, LNCaP-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

67329-1-Ig bindet in WB, IHC, IF, CoIP, ELISA GSK3B und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GSK3B fusion protein Ag17320
• Vollständiger Name: glycogen synthase kinase 3 beta
• Berechnetes Molekulargewicht: 433 aa, 48 kDa
• Beobachtetes Molekulargewicht: 46-48 kDa
• GenBank-Zugangsnummer: BC000251
• Gene symbol: GSK3B
• Gene ID (NCBI): 2932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .

Publikationen

Species	Application	Title
mouse	WB	Mol Nutr Food Res; Douchi Peptides Vy and Sfllr Improve Glucose Homeostasis and Gut Dysbacteriosis In High-Fat Diet-Induced Insulin Resistant Mice; Authors - Songfeng Yu
human	WB,IF	J Inflamm Res; Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3β-Mediated Pathways.; Authors - Wen-Ying Yu
human	WB	Environ Toxicol; Inhibition of FAM83D displays antitumor effects in glioblastoma via down-regulation of the AKT/Wnt/β-catenin pathway.; Authors - Jubo Wang
human	WB,CoIP	J Clin Lab Anal; Circular RNA circβ-catenin aggravates the malignant phenotype of non-small-cell lung cancer via encoding a peptide.; Authors - Weijun Zhao
human	WB	Biomed Res Int; Emodin Alleviates Hydrogen Peroxide-Induced Inflammation and Oxidative Stress via Mitochondrial Dysfunction by Inhibiting the PI3K/mTOR/GSK3β Pathway in Neuroblastoma SH-SY5Y Cells.; Authors - Rui Li
rat	WB	Med Sci Monit; Protective Effects of Ischemic Postconditioning on Livers in Rats with Limb Ischemia-Reperfusion via Glycogen Synthase Kinase 3 beta (GSK-3β)/Fyn/Nuclear Receptor-Erythroid-2-Related Factor (Nrf2) Pathway.; Authors - Qibing Niu