GSK3B Polyklonaler Antikörper

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• KD/KO Validated

GSK3B Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 51065-1-AP

Synonyme

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta

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Galerie der Validierungsdaten

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  WB analysis of A549 using 51065-1-AP

  WB result of GSK3B antibody (51065-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSK3B transfected A549 cells.

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  WB analysis of HeLa using 51065-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51065-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 51065-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51065-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 51065-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51065-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 51065-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51065-1-AP (GSK3B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 51065-1-AP

  IP Result of anti-GSK3B (IP:51065-1-AP, 3ug; Detection:51065-1-AP 1:500) with HeLa cells lysate 920ug.

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  IHC staining of human prostate cancer using 51065-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 51065-1-AP (GSK3B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 51065-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 51065-1-AP (GSK3B antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of NIH/3T3 using 51065-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 51065-1-AP (GSK3B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HeLa using 51065-1-AP

  Immunofluorescent analysis of HeLa cells using 51065-1-AP (GSK3B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

51065-1-AP bindet in WB, IP, IHC, IF, ELISA GSK3B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: glycogen synthase kinase 3 beta
• Berechnetes Molekulargewicht: 433 aa, 48 kDa
• Beobachtetes Molekulargewicht: 46-52 kDa
• GenBank-Zugangsnummer: BC000251
• Gene symbol: GSK3B
• Gene ID (NCBI): 2932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the C-terminal of GSK3B.

Publikationen

Species	Application	Title
human	WB	Cell Death Differ; RPS23RG1 modulates tau phosphorylation and axon outgrowth through regulating p35 proteasomal degradation.; Authors - Dongdong Zhao
mouse	WB	J Steroid Biochem Mol Biol; High glucose impaired estrogen receptor alpha signaling via β-catenin in osteoblastic MC3T3-E1.; Authors - Rui Wang
mouse	IF	Mol Biol Cell; ILK modulates epithelial polarity and matrix formation in hair follicles.; Authors - Alena Rudkouskaya
human	WB	Biochem Biophys Res Commun; N-glycosylation of CD82 at Asn157 is required for suppressing migration and invasion by reversing EMT via Wnt/β-catenin pathway in colon cancer; Authors - Lin Zhu
human	WB	Front Pharmacol; Chebulagic acid suppresses gastric cancer by inhibiting the AURKA/β-catenin/Wnt pathway; Authors - Jing Zhao
human	WB	Int J Dev Neurosci; AQP8 may affect glioma proliferation and growth by regulating GSK-3β phosphorylation and nuclear transport of β-catenin; Authors - Ziling Cai