GRP94 Rekombinanter Antikörper

GRP94 Rekombinant Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

6G15

Kat-Nr. : 82805-2-RR

Synonyme

ECGP, Endoplasmin, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1

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Galerie der Validierungsdaten

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  WB analysis using 82805-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82805-2-RR (GRP94 antibody) at dilution of 1:3200 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 82805-2-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 82805-2-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 82805-2-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 82805-2-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6400
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82805-2-RR bindet in WB, IHC, ELISA GRP94 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GRP94 fusion protein Ag6344
• Vollständiger Name: heat shock protein 90kDa beta (Grp94), member 1
• Berechnetes Molekulargewicht: 92 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: BC066656
• Gene symbol: HSP90B1
• Gene ID (NCBI): 7184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.